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MessagePosté le: ding2018 - Jeu 3 Mai - 05:50 (2018) Répondre en citant

KUALA LUMPUR Chaussures Homme Nike Vapormax Flyknit 2018 Rouge Pas Cher , Dec. 10 (Xinhua) -- Follwing are the drawing results for the Group Stage of the 2014 AFC (Asian Football Confederation) Champions League made at AFC House here on Tuesday:



Esteghlal (Iran)

Al Rayyan (Qatar)

Al Jazira (United Arab Emirates)

Al Shabab (Saudi Arabia)


Al Fateh (Saudi Arabia)

Foolad Khouzestan (Iran)

Winner of Playoff (West)

Bunyodkor (Uzbekistan)


Al Ain (United Arab Emirates)

Al Ittihad (Saudi Arabia)

Tractorsazi Tabriz (Iran)

Winner of Playoff (West)


Al Sadd (Qatar)

Al Ahli (United Arab Emirates)

Al Hilal (Saudi Arabia)

Sepahan (Iran)



Pohang Steelers (South Korea)

Buriram United (Thailand)

Shandong Luneng Taishan (China)

League 3rd place (Japan)


Sanfrecce Hiroshima (Japan)

Central Coast Mariners (Australia)

FC Seoul (South Korea)

Winner of Playoff 3.1 (East)


Guangzhou Evergrande (China)

Knockout Champion (Japan)

Jeonbuk Hyundai Motors (South Korea)

Winner of Playoff (East)


Western Sydney Wanderers (Australia)

Guizhou Renhe (China)

League Runner-up (Japan)

Ulsan Hyundai (South Korea)

SAN FRANCISCO, Sept. 20 (Xinhua) -- Researchers in the United States have identified a key region within a protein that governs how accurately the gene-editing tool known as CRISPR-Cas9 homes in on a target deoxyribonucleic acid (DNA) sequence.

CRISPR is short for Clustered Regularly Interspaced Short Palindromic Repeats and Cas9 stands for CRISPR Associated Protein.

The researchers at the University of California, Berkeley Chaussures Homme Nike Vapormax Bleu Noir Pas Cher , and Massachusetts General Hospital said they have tweaked the region within the Cas9 protein to produce a hyper-accurate gene editor with the lowest level of off-target cutting to date. Identified as a master controller of DNA cutting, the protein domain known as REC3 is a target for re-engineering to further improve accuracy and to minimize the chance that CRISPR-Cas9 will edit DNA at the wrong place.

Co-first author Janice Chen, a graduate student in the lab of Jennifer Doudna Chaussures Homme Nike Vapormax Flyknit 2018 Bleu Pas Cher , the UC Berkeley professor of molecular and cell biology who was one of the first scientists to invent CRISPR-Cas9 technology, noted in a press release that "we have found that even minor alterations in the REC3 domain of Cas9 affect the differential between on- and off-target editing, which suggests that this domain is an obvious candidate for in-depth mutagenesis to improve targeting specificity."

Targeting specificity is a key consideration when doing gene therapy in humans.

Currently Chaussures Nike Vapormax Flyknit 2018 Grise Pas Cher , scientists using CRISPR-Cas9 create a single-guide ribonucleic acid, or sgRNA, an RNA molecule that includes a chain of 20 ribonucleic acids that complements a specific 20-nucleic-acid DNA sequence they want to target Chaussures Homme Nike Vapormax Flyknit 2018 Noir Pas Cher , and attach it to Cas9. This guide RNA allows Cas9 to home in on the complementary DNA, bind to it and cut the double stranded helix.

However, the Cas9-sgRNA complex can bind to DNA that doesn't exactly match Chaussures Homme Nike Vapormax Flyknit Noir Rainbow Pas Cher , leading to undesirable off-target cutting.

Doudna's lab discovered in 2015 a conformational switch of Cas9 that is activated when the RNA guide and DNA target match. They found that only when the RNA and DNA match closely does the 3D structure of Cas9, in particular the conformation of the HNH nuclease domain, change and activate the scissors of Cas9. But the process for sensing the nucleic acids upstream of the conformational switch remained unknown.

In the current study supported by the U.S. National Institutes of Health (NIH) and the U.S. National Science Foundation (NSF) Chaussures Nike Vapormax 2018 Noir Pas Cher , Chen and her colleagues used a technique called single-molecule Forster resonance energy transfer (FRET) to precisely measure how the various protein domains in the Cas9-sgRNA protein complex - in particular REC3, REC2 and HNH - move when the complex binds to DNA.

The researchers uncovered that the REC3 domain is responsible for sensing the accuracy of target binding, which then signals the outward rotation of the REC2 domain to open a path for the HNH nuclease domain Pas Cher Chaussures Nike Vapormax Off-White Blanche , activating the scissors. They then showed that by mutating parts of REC3, it is possible to change the specificity of the Cas9 protein so that the HNH nuclease is not activated unless the guide RNA and target DNA match is very close.

They were able to engineer an improved hyper-accurate Cas9, dubbed HypaCas9 Chaussures Nike Vapormax Off-White Noir AA3831-100 Pas Cher , that retains its on-target efficiency but is slightly better at discriminating between on- and off-target sites in human cells.

"If you mutate certain amino acid residues in REC3, you can tweak the balance between Cas9 on-target activity and improve specificity; we were able to find the sweet spot where there is sufficient activity at the intended target but also a large reduction in off-target events," Chen was quoted as saying in the news release from UC Berkeley.

Xi meets Singaporean PM on advancing ties

Rescue teams work to find survivors as Mexico quake toll reaches 230

In pics: general debate of UN General Assembly on day 2

Highlights of 10th China Acrobatics Golden Chrysanthemum Awards

Highlights of 10th China Acrobatics Golden Chrysanthemum Awards

Wildlife paradise: Changtang National Nature Reserve in China's Tibet

PLA Navy frigates introduced to visitors at Port of Antwerp Nike VaporMax Flyknit Femme Pas Cher , Belgium

Aviation Expo China 2017 held in Beijing

The Netherlands' Judith Pietersen (L) and Yvon Belien react during a women's preliminary match of Volleyball against Serbia at the 2016 Rio Olympic Games in Rio de Janeiro, Brazil, on Aug. 14, 2016. The Netherlands won 3-2. (XinhuaYue Yuewei)

BEIJING, March 10 (Xinhua) -- China's 13th Five-Year Plan concerns more than just i. Wholesale Jerseys   Wholesale Jerseys Free Shipping   Wholesale Jerseys China Free Shipping   Wholesale NFL Jerseys China   Wholesale College Jerseys From China   Wholesale Basketball Jerseys Free Shipping   Cheap College Jerseys   Wholesale New Nike NBA Jerseys   Cheap College Jerseys Wholesale   Wholesale NBA Jerseys 

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